Jamey Wierzbowski

Willow Data Consulting
Greater Boston/Cambridge Area jamey@willowdataconsulting.com

Scientific software developer -consultant with 25 years experience providing informatics solutions for biotech and pharmaceutical research. Jamey has built and integrated many full stack applications for life science research as well as implemented and managed the customization and integration of commerical systems. Willow Data Consulting (Jamey) is looking for contract or full time opportunities to help with your scientific informatics challenges

Experience

Scientific Application Developer in Human Biology and Data Science

Eisai Co., Ltd.

Developer and Data Science Engineer in the Human Biology and Data Science informatics group:
-Developed tools for processing, querying, exporting and visualizing genetic association and cheminformatic operational big data
-JAVA , Python, Javascript, Pipeline Pilot, RESTful web application development with both relational and noSQL databases on Tomcat, Apache and Flask servers
-Developed mongoDB data repository aligning whole genome and whole exome disease association PLINK statistics and variant consequences for over 12K individuals in multiple association tests
-Developed a human genome Target Gene Explorer that integrated various disease association data, expression, variant consequences and epigenetic databases and file types for the disease of interest
-Automated data gathering and reporting of numerous public genomic datasources
-A development team member for a Target Gene Notebook web application to record genetic findings and manage publications.
-Built a human disease variant data processing pipeline using Python and executables including Plink, vcftools, snpEff
-Developed data visualizations with open source packages in Java, Javascript, Python and R Shiny
-Data engineering for registration, processing, inventory management and analysis of laboratory high throughput data and materials
-Built and supported a LIMS- compound and biological archive including integration of various robotic equipment
-Developed compound, extract, siRNA, physchem, DMPK data registries and associated data loading and viewing web application tools
-Evaluation, integration, configuration and support of various commercial scientific platforms for high throughput screening and lab robotics.
-Configuration and support of Chemcart, Certara D360, Sapphire Biobank installations

Dec 2007 – Dec 2018

Clinical Informatics Software Engineer

Gulfstream Bioinformatics Corporation


-Built data management tools that were components of a novel biomedical information “Knowledge capture” system. This involved coding extensive PERL applications that process complex relational spreadsheet and biomedical database information.
-Developer for “BIGR”, a validated JAVA enterprise application for biomaterials and information for genomic research. Enterprise Struts/EJB architecture with over 3000 class files. Developed and supported all layers of this dynamic application for managing biological specimens and associated clinical annotation. .

March 2007 – Dec 2007

Senior Research Scientist

Cellicon Biotechnologies, Inc. (Boston University)


-Developed a microbial gene expression microarray analysis platform for systems biology research. This included relational database design and management with MySQL. Developed front end web application analysis tools using JDBC, SQL, J2EE, JSP, JAVA Servlets. Produced statistical and visual analysis applications using JAVA integrated MATLAB
-Developed LIMS system for high-throughput screening of chemical libraries against antimicrobial targets and pathogenic organisms.
-Designed and maintained company website and internal knowledge web server .

Dec. 2003 – Feb. 2007

Senior Associate in Research & Development

Agencourt Bioscience Corp.


-Built a protein interaction information system. This involved Oracle database design, JDBC applications, Java servlets, and graphical display JAVA applets
-Designed environmental sample microbial DNA analysis system. Automated the BLAST analysis, annotation and display of genes identified from environmental samples

Jan. 2002 – Nov. 2003

Bioautomation Scientist

Whitehead Institute Center for Genomic Research


Software development duties included building and designing the following:
-JAVA and PERL based executables for the human genome project quality control
-Co-supervisory role for the development team .

1999 - 2002

Scientist I- IV

Genome Therapeutics, Corp.


Scientist for various genomic analysis projects .

1993- 1999

Education

Brandeis University

Master of Software Engineering
May 2004

Harvard University

Master of Liberal Arts, Biology
June 1999

SUNY College at Buffalo

Bachelor of Arts, Biology
May 1993

Skills

Programming Languages & Tools
Technical Expertise
  • Languages - JAVA, Python, JavaScript, SQL, R, Perl
  • Databases - Oracle, MySQL, MongoDB, SQLite, SQL server, Access
  • Applications - Pipeline Pilot, Docker, GIT, Chemaxon, VCFtools, SAMtools, R Shiny, Jupyter Notebook
  • Web Servers - Tomcat, JBoss, Apache, Flask, Spark Java
  • Frameworks - JQueryUI, Maven, Spring, POJO, JSP/ Servlets, Java Web Start, RESTful Web Services
  • Concepts - bioinformatics, clinical informatics, genomics, target gene reporting, cheminformatics, LIMS, robotic automation, reproducible research, version control, software development life cycle, compliance validation, EC2 cloud computing, application containerss
  • OS - Linux, Windows
  • IDE - Eclipse, PyDev, Websphere, Netbeans, Visual Studio, R studio, SQL developer, TOAD

Publications

  • Open full list Google Scholar
  • Kohanski MA, Dwyer DJ, Wierzbowski J, Cottarel G, Collins JJ. Mistranslation of membrane proteins and two-component system activation trigger antibiotic-mediated cell death. Cell. 2008 Nov 14;135(4):679-90
  • Jeremiah J Faith , Boris Hayete , Joshua T Thaden , Ilaria Mogno , Jamey Wierzbowski , Guillaume Cottarel , Simon Kasif , James J Collins , Timothy S Gardner. 2007. Large-Scale Mapping and Validation of Escherichia coli Transcriptional Regulation from a Compendium of Expression Profiles. PLoS Biology. 5(1). 54-66.
  • Malek, J.A., Wierzbowski, J.M. , Tao, W., Bosak, S.A., Saranga, D.J., Doucette-Stamm, L., Smith, D.R., McEwan, P.J. & McKernan, K.J.. 2004. Protein interaction mapping on a functional shotgun sequence of Rickettsia sibirica. Nucleic Acids Research. 32(3). 1059-1064.
  • Waterston, R.H., et. al. 2002. Initial sequencing and comparative analysis of the mouse genome. Nature 242:520-562.
  • Lander, E.S. et al. 2001 (Supplemental author list). Initial sequencing and analysis of the human genome. Nature 409: 860-920.
  • Smith et al., 1997. Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics. J Bacteriol 179(22):7135-55.
  • Auger, S.R., Thomann, U., Oriss, D., Ayers, M. , Wierzbowski, J., Mao, J., 1997. Automated hybridization and imaging for chemiluminescence-based multiplex sequencing. SPIE Proceedings 2985:145-152.

Patents

  • RecA Inhibitors with Antibiotic Activity, Compositions and Methods of Use Patent date Issued Nov 20, 2008
  • COMPOSITIONS AND METHODS FOR POTENTIATING ANTIBIOTIC ACTIVITY Patent date Filed Sep 16, 2010
  • COMPOSITIONS AND METHODS TO POTENTIATE COLISTIN ACTIVITY Patent date Filed Feb 4, 2010
  • Devices and methods for simultaneous separation and purification of molecular samples Patent date Filed Nov 14, 2002
  • Compositions and Methods for Antibiotic Potentiation and Drug Discovery Patent date Filed Nov 14, 2002

Demos